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Defined minimal medium for a thermophilicBacillus sp.developed by a chemostat pulse and shift technique

✍ Scribed by H. Kuhn; U. Friederich; A. Fiechter

Publisher: Springer
Year: 1979
Tongue: English
Weight: 388 KB
Volume: 6
Category: Article
ISSN: 1432-0614
DOI: 10.1007/bf00499164

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✦ Synopsis

A new method for the design and optimization of a minimal medium is described. A chemostat pulse technique was used to identify growth limiting nutrients. By combining this pulse technique with medium shifts, the essential medium components were determined and the composition quantified. The described technique has useful applications for fastidious organisms and organisms which cannot be easily cultivated in shake flasks. A minimal medium is given for thermophilic Bacillus caldotenax, which exhibits methionine and biotin auxotrophy.

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