Defective prophage in Escherichia coli K12 strains
β Scribed by Ann Strathern; Ira Herskowitz
- Book ID
- 119009117
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 721 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0042-6822
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If ultraviolet irradiated, lambda-lysogenic Escherichia coli K12 bacteria are incubated for 4 to 6 h at 30 degrees C, lambda prophage becomes inactive in the non-surviving cells. This was demonstrated by the use of lambda cIts857ind1 prophage which can be induced by heat but not by ultraviolet light
Two routes of one-carbon biosynthesis have been described in Escherichia coli K12. One is from serine via the serine hydroxymethyltransferase (SHMT) reaction, and the other is from glycine via the glycine cleavage (GCV) enzyme system. To isolate mutants deficient in the GCV pathway, we used a select