Defective membrane expression of the LFA-1 complex may be secondary to the absence of the β chain in a child with recurrent bacterial infection
✍ Scribed by Barbara Lisowska-Grospierre; Marie-Christine Bohler; Alain Fischer; Claude Mawas; Thymothy A. Sringer; Claude Giscelli
- Book ID
- 102160787
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 486 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Defective membrane expression of the LFA-1 complex may be secondary to the absence of the p chain in a child with recurrent bacterial infection* Membrane and intracellular processing of the LFA-1 macromolecular complex, known to be involved in cytolytic function of T lymphocytes, was investigated in a child with recurrent bacterial infections, impaired natural killer activity, T cell-mediated lymphocytolysis and absent adhesion and migration of phagocytic cells. Monoclonal antibodies to the LFA-1 a and / 3 subunits, able to precipitate the LFA-la, 180-kDa chain, the p151 chain and fi 94-kDa chain (shared by both a chains), were used in immunoprecipitation studies of patient and control phytohemagglutinin-blasts.
Neither of the a chains nor the fi chain were found in precipitates obtained from 1251surface-labeled patient cells in contrast to controls. However, the precursor of the LFA-1 a chain, a 170-kDa polypeptide, was identified in lysates of biosynthetically labeled patients' cells. These results suggest that the defective membrane expression of the LFA-1 complex may be secondary to the absence of the mature p chain.