Defective human T-lymphotrophic virus type I provirus in T-cell prolymphocytic leukaemia
β Scribed by Kensuke Kojima; Takashi Sawada; Takayuki Ikezoe; Yoshinobu Matsuo; Hajime Kobayashi; Tomofumi Yano; Takeshi Sugimoto; Shion Imoto; Toshitaro Nakagawa; Toshimitsu Matsui; Masaki Yasukawa; Masamichi Hara; Hirokuni Taguchi
- Book ID
- 108672233
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 161 KB
- Volume
- 105
- Category
- Article
- ISSN
- 0007-1048
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## Abstract Adult Tβcell leukaemia (ATL) is a neoplastic disease derived from CD4^+^ Tβlymphocytes and etiologically associated with human Tβcell leukaemia virus type I (HTLVβI). In addition to structural genes, HTLVβI encodes regulatory and accessory genes in the pX region. Among them, Tax is thou
For the detection of the HTLV-I provirus genomic DNA, we applied the polymerasechain-reaction (PCR) dot-blot assay system developed by Saiki et al. (14). DNA sequences coding the p24 in the gag region were chosen as targets to be amplified. We checked genomic DNAs extracted from peripheral blood lym