Decreased tumorigenicity of a transplantable rat sarcoma following transfer and expression of an IL-2 cDNA
✍ Scribed by Stephen J. Russell; Suzanne A. Eccles; Claudia L. Flemming; Caroline A. Johnson; Mary K. L. Collins
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- French
- Weight
- 940 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
The notion that tumour-cell-derived IL-2 might lead to paracrine stimulation of the host anti-tumour response was tested in a transplantable rat sarcoma model. Three HSNLV clones induced to secrete different amounts of human IL-2 following retroviral gene transfer showed reduced tumorigenicity and metastatic potential in athymic (nulnu) and immunocompetent syngeneic rats which was directly related to the level of IL-2 secretion. In contrast, the tumorigenicity of HSNLV clones secreting a biologically inactive form of IL-2 (IL-2Lyszo) was unaltered. T-lymphocyte-mediated rejection of Zip I, the highest IL-2 producer, was demonstrated histologically in hooded rats and infiltrating mononuclear cells were also observed in Zip1 tumours growing in athymic rats. Tumours derived from IL-2-secreting HSNLV showed reduced or absent IL-2 secretion in immunocompetent rats, sometimes with associated loss of the IL-2 provirus, but continued to secrete IL-2 in nude rats. The host response to Moloney-helper-virus-infected HSNLV was also examined and the results represent a cautionary note to those undertaking experiments of a similar nature. 3To whom correspondence and reprint requests should be addressed.