Decreased cytoplasmic immunoglobulin a production during epstein-barr virus immortalization on lymphocytes from patients with ataxia-telangiectasia
✍ Scribed by Motohiko Okano; Yukio Sakiyama; Shuzo Matsumoto; Fumio Mizuno; Toyoro Osato
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 287 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0887-8013
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✦ Synopsis
Previously, we have reported significantly lower immunoglobulin (lg) A production in supernatants of cultured lymphoblastoid cells using enzyme-linked immunosorbent assay from patients with ataxia-telangiectasia (AT) when compared to that of age-and sexmatched healthy individuals. Here, we further assess the degree of cytoplasmic lg production in these cells and also analyze rt during the early phase of Epstein-Barr virus immortalization. All classes of cytoplasmic IgM, IgG, and IgA productions were demonstrated in cells from healthy controls. In contrast, cells from patients withATshowed only cytoplasmic IgM and IgG with low or nondetectable levels of IgA during and after the immortalizing process. These results suggest B lymphocytes bearing IgAare functionally immature and/or defective in patients with AT. o 1995 Wiiey-Liss, inc