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Decreased 3H-uridine incorporation and increased 3H-adenosine incorporation by hela cells exposed to autologous culture fluid

✍ Scribed by Roger Tilley; C. N. Nair

Book ID: 102883603
Publisher: John Wiley and Sons
Year: 1975
Tongue: English
Weight: 702 KB
Volume: 86
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040860408

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✦ Synopsis

Actively growing HeLa monolayer cultures briefly exposed to the culture fluids (CF) from confluent HeLa cultures and labeled simultaneously or subsequently, incorporated less 3H-uridine ( 3H-UR ) but more 3H-adenosine ( 3H-AR) than control cultures similarly exposed to fresh medium and labeled. Exposure to CF inhibited the uptake as well as the incorporation of 3H-UR by cultures. The inhibition of 3H-UR incorporation by CF-exposed cultures could be reduced by increasing the concentration of 3H-UR in the labeling medium. Both the inhibition of 3H-UR incorporation and the stimulation of 3H-AR incorporation were

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