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Decomposition of phosphatidylethanolamine with phospholipase C from Clostridium perfringens

✍ Scribed by É. V. Dyatlovitskaya; V. I. Volkova; M. V. Ispolatovskaya; L. D. Bergel'son


Book ID
112435502
Publisher
Springer
Year
1967
Tongue
English
Weight
217 KB
Volume
16
Category
Article
ISSN
1573-9171

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A fluorescent assay for Clostridium Perfringens phospholipase C is described using l-palmitoyl-2-[6(pyren-l-yl)hexanoyl]-snglycero-3-phospho-N-(trinitrophenyl)aminoethanol (PPHTE) as the substrate. This method is based on the decrease of the quenching of pyrene monomer fluorescence when phospholipas