Decolourization of reactive azo dyes by transformation with Pseudomonas luteola

✍ Scribed by T.L. Hu

• Book ID: 103982558
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 500 KB
• Volume: 49
• Category: Article
• ISSN: 0960-8524
• DOI: 10.1016/0960-8524(94)90172-4

✦ Synopsis

A bacterium isolated from sludge from dyeing wastewater treatment which removed the colour of reactive azo dyes such as Red G, RBB, RP2B and ~RP was identified as P. luteola. After shaking incubation for 48 h, P. luteola removed the colour of these dyes during a further 2 days of static incubation, and the fraction of decolourization was 37"4% (Red G), 93"2% (RBB), 92"4% (RP2B) and 88% (V2RP). There was no increased removal of colour when P. luteola was cultured in a low-N medium. No colour was removed under continuous shaking or completely static incubation. According to results from a thin-layer chromatogram, Red G was not degraded and reduction of the azo bond (--N=N--) was the major reason for its decolourization. An extra two spots appeared on the TLC for the other three reactive dyes, which indicated that they were degraded by E luteola; the azoreductase activity was high when RBB was the substrate, but sulphanilic acid was not found in the metabolites.