## Abstract This study attempted to investigate whether different levels of mitotic activity exist within different physical regions of a human embryonic stem (hES) cell colony. Incorporation of 5‐bromo‐2‐deoxyuridine (BrdU) within newly‐synthesized DNA, followed by immunocytochemical staining was
✦ LIBER ✦
Deciphering the Importance of Three Key Media Components in Human Embryonic Stem Cell Cultures
✍ Scribed by Vanessa Ding; Andre B. H. Choo; Steve K. W. Oh
- Publisher
- Springer Netherlands
- Year
- 2006
- Tongue
- English
- Weight
- 275 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0141-5492
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## Abstract In this study a three step culture system, 2D‐3D sequential culture __in vitro__ and further implantation __in vivo__ was developed to induce human embryonic stem cells (hESCs) into cartilage like tissues. Five‐day‐old embryoid bodies were plated for chondrogenic induction for 27 days (