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Decarboxylation of [1-13C]Leucine by Peroxyl Radicals

โœ Scribed by F. Tinardon; R. Lamrini; P. Lacan; M. Desage; A. Francina

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
120 KB
Volume
11
Category
Article
ISSN
0951-4198

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โœฆ Synopsis

The decarboxylation of [1-13 C]leucine by peroxyl radicals (ROO โ€ข ) has been studied by using combined gas chromatography/isotope ratio mass spectrometry (GC/IRMS) to follow the production of 13 CO 2 . Peroxyl radicals were obtained by thermal decomposition of a water soluble azo initiator, 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH). The decarboxylation rates measured by GC/IRMS were dependent on [1-13 C]leucine and AAPH concentrations. The leucine partial order was found to be constant over a 60 min. period (0.6) but the AAPH partial order was found to be variable during the same period, from 1.0 (t = 0) to 0.50 (t = 60 min.). The identification of the products of reaction between leucine and peroxyl radicals requires the use of combined gas chromatography/mass spectrometry using the selected-ion monitoring mode. Methyl-3 butanoate was identified from comparison of its mass spectrum with that of a standard.

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