𝔖 Bobbio Scriptorium
✦   LIBER   ✦

DBcAMP effect on the expression of GFAP and of its encoding mRNA in astroglial primary cultures

✍ Scribed by G. Le Prince; C. Fages; B. Rolland; J. Nunez; M. Tardy

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
791 KB
Volume
4
Category
Article
ISSN
0894-1491

No coin nor oath required. For personal study only.

✦ Synopsis

Short term and chronic dBcAMP effects on the expression of glial fibrillary acidic protein (GFAP) in astroglial primary cultures are investigated. Short (48 h) and long (more than 7 days) treatments with the CAMP derivative induce both cell shape changes and an increase in GFAP immunolabelling. Such effects are only associated with an increase in GFAP and in GFAP-mRNA levels in the long term treatment. These results suggest that the short term effect of dBcAMP induces post-translational modifications of the protein whereas the long term effect is associated with an increase in GFAP mRNA transcription andor stability.

📜 SIMILAR VOLUMES

Effect of growth factors and steroids on
Effect of growth factors and steroids on transglutaminase activity and expression in primary astroglial cell cultures
✍ A. Campisi; V. Bramanti; D. Caccamo; G. Li Volti; G. Cannavò; M. Currò; G. Racit 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 485 KB

## Abstract Type‐2 transglutaminase (TG‐2) is a multifunctional enzyme involved in the regulation of cell differentiation and survival that recently has been shown to play an emerging role in astrocytes, where it is involved in both proliferation and differentiation processes. Growth factors (GFs)

The effect of dehydroepiandrosterone on
The effect of dehydroepiandrosterone on lipogenic gene mRNA expression in cultured primary chicken hepatocytes
✍ Xue Tang; Haitian Ma; Guoqing Huang; Jinfeng Miao; Sixiang Zou 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 434 KB 👁 1 views

## Abstract The effects of dehydroepiandrosterone (DHEA) on lipogenic gene mRNA expression were examined using cultured primary chicken hepatocytes. Cell samples were equilibrated to culture conditions for 24 h and then exposed to DHEA (1, 10 and 100 µM) dissolved in DMSO. The expression of sterol

Effect of phenytoin on cytochrome P450 2
Effect of phenytoin on cytochrome P450 2B mRNA expression in primary rat astrocyte cultures
✍ Bernd Ibach; Kurt Appel; Peter Gebicke-Haerter; Ralf Peter Meyer; Thomas Friedbe 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 242 KB 👁 2 views

Studies on cytochrome P450 2B (CYP2B) in the brain have essentially been focused on protein characterization and regional distribution. Due to the high sequence homology between the closely related CYP2B1 and 2B2 isoforms and the low amounts of the corresponding mRNAs few efforts have been made to a

Effect of silybin and its glycosides on
Effect of silybin and its glycosides on the expression of cytochromes P450 1A2 and 3A4 in primary cultures of human hepatocytes
✍ Pavel Kosina; Patrick Maurel; Jitka Ulrichová; Zdeněk Dvořák 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 203 KB 👁 1 views

## Abstract Four β‐glycosides of flavonoligan silybin, i.e. silybin β‐galactoside, silybin β‐glucoside, silybin β‐maltoside, silybin β‐lactoside were synthesized in order to improve silybin water solubility and bioavailability (Křen et al., J Chem Soc, Perkin Trans 1, 2467–2474, 1997). The presente

Effect of epidermal growth factor on the
Effect of epidermal growth factor on the labeling of the various RNA species and of nuclear proteins in primary rat astroglial cell cultures
✍ R. Avola; D. F. Condorelli; L. Turpeenoja; F. Ingrao; S. Reale; N. Ragusa; A. M. 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 886 KB

This study investigated the effects of epidermal growth factor (EGF) on the labeling of various RNA species and of nuclear proteins in primary rat astroglial cell cultures. After 12 hours of EGF treatment in serumfree medium or chemically defined medium, significant increase in RNA labeling, and als