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Dark fermentative bio-hydrogen production from waste wheat starch using co-culture with periodic feeding: Effects of substrate loading rate

✍ Scribed by Serpil Ozmihci; Fikret Kargi


Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
415 KB
Volume
36
Category
Article
ISSN
0360-3199

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✦ Synopsis


Dark fermentation experiments were performed for bio-hydrogen production from ground wheat starch solution (10 AE 1 g l À1 ) using periodic feeding and effluent removal. A mixed culture of Clostridium butyricum-NRRL 1024 and Clostridium pasteurianum-NRRL B-598 were used with an initial biomass ratio of 1/1.Effects of wheat starch loading rate on the rate and yield of bio-hydrogen formation were investigated. Substrate loading rate was varied between 0.54 and 5.52 g d À1 (HRT ¼ 6e60 h). The highest hydrogen formation rate (280 ml d À1 ), volumetric hydrogen formation rate (1857 ml H 2 l À1 d À1 ) and volatile fatty acids (VFAs) concentration were obtained with a substrate loading rate of 5.52 g d À1 (HRT ¼ 6 h). The highest hydrogen yield (109 ml H 2 g TS À1 ) was obtained with a substrate loading rate of 1.38 g d À1 .