d-Lactic acid in pork as a freshness indicator monitored by immobilized d-lactate dehydrogenase using sequential injection analysis

✍ Scribed by Hun-Chi Shu; H»kan H»kanson; Bo Mattiasson

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 715 KB
• Volume: 283
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85287-t

✦ Synopsis

A sequential injection analysis system was set up to quantify u-lactic acid in pork. o-Lactic acid seems to be a useful indicator of metabolism of spoilage organisms . The analytical system is fully computerized and is able to monitor the concentration of the acid in an assay cycle of 160 s including 90 s incubation time when the flow is stopped. The enzyme used in the analysis is D-lactate dehydrogenase that is co-immobilized with L-alanine aminotransferase on porous glass . The experimental set-up gave good reproducibility down to 0 .1 mM n-lactate and the assay could be repeated at least 500 times with the response maintained .