D-glucose transporter on mussel mantle cell membranes: Effect of sodium and phlorizin

✍ Scribed by Louzao, M. C. ;Vieytes, M. R. ;Botana, L. M.

• Book ID: 102891982
• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 506 KB
• Volume: 267
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402670604

✦ Synopsis

Abstract

We studied the kinetics of the glucose transporter in mussel mantle cells. The transport is sodium dependent, with a very low uptake of glucose when sodium is replaced with potassium, choline or sucrose. In the presence of phlorizin, a glucose transporter inhibitor, the uptake is greatly diminished, with a low glucose uptake which is probably due to passive diffusion. The Woolf‐Augustinsson‐Hofstee plot obtained with different concentrations of glucose, and the Hill coefficient obtained in studies about the effect of Na^+^ on glucose uptake, indicate that more than one sodium ion was involved in the transport of each molecule of glucose across the membrane in mussel mantle cells, or that positive cooperativity is occurring. The activity of the transporter is not affected by the sodium‐proton pump inhibitor amiloride. © 1993 Wiley‐Liss, Inc.