CZE separations of basic proteins at low pH in fused silica capillaries with surfaces modified by silane derivatization and/or adsorption of polar polymers
✍ Scribed by Gilges, Martin ;Husmann, Heribert ;Kleemiß, Maria-Helen ;Motsch, Stephan R. ;Schomburg, Gerhard
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 487 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
Mixtures of several basic proteins have been used to test CZE capillaries with surfaces modified by new pretreatment procedures; the performance obtained has been compared with that achieved using capillaries treated by procedures described in the literature.
It has been shown that addition of non‐ionic polyvinylalcohols (PVA) to CZE buffer solutions deactivates even bare, i.e. untreated, fused silica surfaces and renders them suitable for separations of basic proteins. The performance obtained from such surfaces was comparable with that of capillaries modified by the more elaborate procedures of etching, silanol derivatization, and/or adsorptive coating (again with polymers).
A home‐made device is described which enables derivatization and coating reactions to be performed on fused silica capillaries under an inert atmosphere, i.e. one free from oxygen and water.