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Cytotoxicity tests for high-throughput drug discovery

โœ Scribed by Kevin Slater

Book ID
104361549
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
51 KB
Volume
12
Category
Article
ISSN
0958-1669

No coin nor oath required. For personal study only.

โœฆ Synopsis

Despite theoretical obstacles associated with performing cell-based assays in high-density formats (microplates with at least 384 wells), it is becoming clear that the pharmaceutical industry is now routinely running cell-based tests in these formats. This work is revealing the weakness of established cytotoxicity end points, specifically in relation to sensitivity and reproducibility. New assay kits based on bioluminescent detection of ATP and ADP are now providing the answer to these problems.

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