Cytotoxicity of trichothecene mycotoxins isolated fromFusarium sporotrichioides(MC-72083) andFusarium sambucinumin baby hamster kidney (BHK-21) cells
✍ Scribed by Lucy H. Senter; Dale R. Sanson; David G. Corley; Michael S. Tempesta; Audrey A. Rottinghaus; George E. Rottinghaus
- Book ID
- 104775837
- Publisher
- Springer Netherlands
- Year
- 1991
- Tongue
- English
- Weight
- 437 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0301-486X
No coin nor oath required. For personal study only.
✦ Synopsis
Twenty-six trichothecene mycotoxins produced by Fusarium sporotrichioides (MC-72083) and Fusarium sambucinum were screened for relative cytotoxicity in cultured baby hamster kidney (BHK-21) cells. The relative cytotoxicity was measured as LClo o. The most cytotoxic trichothecenes were T-2 toxin (5 ng/ml) and the recently isolated 4-propanoyl HT-2 (5 ng/ml) and 3'-hydroxy T-2 toxin (5 ng/ml). T-2 tetraol (1 x 104 ng/ml), 8-/~-hydroxytrichothecene (1 x 104 ng/ml), sporotrichiol (2 x 104 ng/ml), 8-oxodiacetoxyscirpenol (6 x 104 ng/ml) and 8-acetyl T-2 tetraol (1 x 105 ng/ml) were the least toxic of the regular trichothecenes. None of the modified trichothecenes or the apotrichothecene were very cytotoxic: 8-/~hydroxysambucoin (2 x 103 ng/ml), FS-1 (5 x 103 ng/ml), 8-e-hydroxysambucoin (8 x 104 ng/ml) and trichotriol (1 x 105 ng/ml). The modified trichothecenes, FS-2 and FS-3, were not toxic even at 1 x 105 ng/ml. The baby hamster kidney cell bioassay proved to be a very sensitive and reproducible means of screening new trichothecene mycotoxins for relative cytotoxicity.