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✦   LIBER   ✦

Cytotoxicity of sera from patients with scleroderma

✍ Scribed by Stanley Cohen; Dr. Alice R. Johnson; Eric Hurd

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
847 KB
Volume
26
Category
Article
ISSN
0004-3591

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✦ Synopsis

Abstract

Sera from patients with progressive systemic sclerosis were compared with sera from normal individuals and from patients with other connective tissue diseases for cytotoxic effects on cultured human cells. More than 40% of the sera from patients with active progressive systemic sclerosis were cytotoxic by several criteria for pulmonary arterial or umbilical venous endothelial cells, foreskin fibroblasts, and neuroblastoma cells. Cytotoxic sera caused morphologic changes, uptake of trypan blue dye, and a decrease in the incorporation of ^3^H‐thymidine into DNA. In contrast, only 4 sera from normal individuals or patients with other rheumatic diseases affected cell morphology, staining, or uptake of ^3^H‐thymidine. Partial characterization of the cytotoxic factor indicated that it is sensitive to proteolysis by trypsin. The molecular weight of the factor was estimated to be similar to that of albumin.

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