Cytotoxicity and mutagenicity of 6-aminonicotinamide in Chinese hamster ovary cells

✍ Scribed by F. Sanz; J. M. Pozuelo; A. Santa Maria

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 340 KB
• Volume: 16
• Category: Article
• ISSN: 0270-3211
• DOI: 10.1002/(sici)1520-6866(1996)16:1<19::aid-tcm3>3.0.co;2-j

✦ Synopsis

A strong teratogen-6-aminonicotinamide (6-AN)-was tested for its ability to induce cytotoxicity and mutagenicity in Chinese hamster ovary (CHO) cells. Tests were performed in the presence and absence of a metabolic activation system (S-9 mix). Cytotoxicity was evaluated in CHO cells by the total protein content. The two singlegene mutation systems in CHO cells have been investigated. Both involve evaluating the response of the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) locus and specific inhibitors of the cellular (Na++K+)-ATPase using 6-thioguanine and ouabain as selective agents, respectively. From our results, 6-AN showed a higher cytotoxic effect at concentrations over 1 x lo-' mg/ml. Cytotoxicity was significantly different with and without S-9 mix. 6-AN was cytotoxic per se, however, when 6-AN was biotransformed, in the presence of S-9 mix no biological activity (cytotoxic) was detected. Non-significant mutagenic activity was detected with 6-AN in the presence and in the absence of the metabolic activation System.