Cytotoxic T lymphocyte responses of infants after natural infection or immunization with live cold-recombinant or inactivated influenza A virus vaccine
✍ Scribed by Mbawuike, Innocent N.; Piedra, Pedro A.; Cate, Thomas R.; Couch, Robert B.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 751 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0146-6615
No coin nor oath required. For personal study only.
✦ Synopsis
The cytotoxic T lymphocyte (CTL) response of infants after immunization with either inactivated trivalent subvirion vaccine (TIV) or bivalent attenuated cold-recombinant (CR) vaccine or occurrence of natural influenza virus infection were compared in a blinded, placebo-controlled study during the 1987-1988 and 1988-1989 influenza epidemic seasons. Healthy infants between 6 and 13 months of age were randomly assigned and administered a single dose of intranasal bivalent (A/H3N2/A/HI N1) CR vaccine, a two-dose regimen of TIV (A/H3N2/A/HI N 1/B) influenza vaccine, or placebo. Peripheral blood lymphocytes were obtained prior to and 2-8 weeks after vaccination and at the end of the epidemic season and stimulated with virus i n vitro for 6 or 7 days. Lysis of autologous virus-infected target cells was assessed in a 4 hr T r release assay. MHC class I-restricted influenza A-specific CTL was stimulated following natural influenza Avirus infection but not after immunization with CR influenza A virus vaccine or TIV. These results demonstrate for the first time induction of influenza virusspecific CTL activity in infants under 1 year of age. 0 1996 Wiley-Liss, Inc.