Cytotoxic response of cloned tumour infiltrating lymphocytes to autologous human glioblastoma
β Scribed by M.C. Kuppner; M.F. Hamou; N. de Tribolet
- Book ID
- 119107016
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 56 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0165-5728
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Peripheral blood lymphocytes (PBL) of a patient with metastatic melanoma were cultured with autologous melanoma cells (Auto-Me) and recombinant interleukin 2 (IL-2) (MLTC-PBL). Thirty-five days later, when no cytotoxicity against Auto-Me or K562 was detectable, MLTC-PBL were cloned in the presence o
## Abstract Peripheral blood lymphocytes of cancer patients were sensitized __in vitro__ to autologous tumour cells in mixed lymphocyteβtumour culture (MLTC). Blast cells were isolated on discontinuous Percoll gradients from MLTC which showed significant stimulation of [<3>H]βthymidine incorporatio
## Abstract Peripheral blood lymphocytes (PBL) from a melanoma (Me) patient, previously shown to be unable to react against the autologous tumor (Me 28) after mixed lymphocyteβtumor culture (MLTC), were cultured __in vitro__ with the autologous tumor in MLTC and/or with ILβ2βcontaining supernatants
Peripheral blood lymphocytes from five patients were stimulated initially in mixed lymphocyte:tumour culture (MLTC) with autologous malignant effusions and cloned by limiting dilution in interleukin-2 prior to phenotyping and assay for different functional capabilities namely proliferative responsiv