Cytotoxic Evaluation of Pungencine: A New Tropane Alkaloid from the Roots of Erythroxylum pungens O. E. Schulz

✍ Scribed by José G. Sena-Filho; Marcelo S. da Silva; Josean F. Tavares; Steno L. Oliveira; Marco A. V. Romero; Haroudo S. Xavier; Jose M. Barbosa-Filho; Raimundo Braz-Filho

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: German
• Weight: 141 KB
• Volume: 93
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/hlca.200900434

✦ Synopsis

Abstract

A new tropane alkaloid as well as a new substitution group, (3‐endo,8‐anti)‐8‐methyl‐8‐azabicyclo[3.2.1]oct‐3‐yl 4‐hydroxy‐3,5‐dimethoxybenzoate, called pungencine (1), was isolated from the roots of Erythroxylum pungens O. E. Schulz. The structure was elucidated by spectral analyses, including ^1^H‐ and ^13^C‐NMR and 2D‐NMR techniques (^1^H,^1^H‐COSY, NOESY, DEPT, HMQC, and HMBC) and HR‐ESI‐MS. Furthermore, compound 1 was tested for cytotoxicity against several cell lines (Jurkat, HL‐60, U937, K562, KG‐1, and U266) and was determined not to inhibit cell viability at 10 μM.