๐”– Bobbio Scriptorium
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Cytotoxic activity of guinea-pig lymph-node cells stimulated in vitro

โœ Scribed by Amnon Altman; Herbert J. Rapp

Publisher
John Wiley and Sons
Year
1978
Tongue
French
Weight
614 KB
Volume
22
Category
Article
ISSN
0020-7136

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โœฆ Synopsis

Abstract

Guineaโ€pig lymphโ€node cells (LNC) cultured for 5 days in medium containing fetal calf serum (FCS) were cytotoxic to various target cells. LNC cultured in medium supplemented with fresh, autologous guineaโ€pig serum (GPS) instead of FCS were not detectably cytotoxic unless agents that stimulate lymphocyte proliferation were added to the culture medium. The stimulating agents we studied were 2โ€mercaptoethanol (2โ€ME), syngeneic tumor cells, allogeneic peritoneal exudate cells (PEC) and the Tโ€cell mitogens concanavalin A (Con A) and phytohemagglutinin (PHA). LNC cultured in the presence of these agents were cytotoxic to normal syngeneic fibroblasts and to syngeneic, allogeneic or xenogeneic tumor cells but not to PHAโ€induced lymphoblasts. Potentiation of cytotoxicity in vitro was accompanied by a marked proliferation of the cultured LNC; the combination of several stimulatory agents had an additive effect on the generation of cytotoxicity in culture.

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