Cytotoxic action of prostaglandin D2 on mouse neuroblastoma cells

✍ Scribed by Haruhiro Higashida; Kyoko Kano-Tanaka; Shunnosuke Natsuume-Sakai; Katsuko Sudo; Hiroko Fukami; Yuzo Nakagawa; Naomasa Miki

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: French
• Weight: 709 KB
• Volume: 31
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910310621

✦ Synopsis

Addition to the culture medium of prostaglandin (PG) D, resulted in the degeneration in a dose-and time-dependent manner of N18TG-2 cells cloned from mouse neuroblastoma. The EDso for PGD,-induced cytotoxicity was about 10 1(~. The degenerative changes were irreversible when the cells were exposed for more than 10 h. Scanning and transmission electron microscopic examination revealed that treatment with PGD, resulted in apearance of numerous blebs of various sizes along the cell surface and also in destruction of surface membrane and of cytoplasmic organelles. Tumor weight of N 18TG-2 neuroblastoma inoculated subcutaneously on the backs of A/J mice was about 35-70 YO less than that of controls after 14 days of single daily i.p. or S.C. injections of 0.5-1 mg/kg of PGD,. The results indicate that PGD, has growth-inhibitory effects on mouse neuroblastoma cells in vitro and in vivo.