✦ LIBER ✦

Cytosolic ionized calcium concentration in isolated chondrocytes from each zone of the growth plate

✍ Scribed by Dr. J. P. Iannotti; C. T. Brighton

Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 736 KB
Volume: 7
Category: Article
ISSN: 0736-0266
DOI: 10.1002/jor.1100070408

No coin nor oath required. For personal study only.

✦ Synopsis

In isolated chondrocytes from the growth plate, there is an increase in both the cytosolic ionized calcium concentration and in total cell calcium content as the cells approach the mineralization front. The reserve zone chondrocytes have a cytosolic ionized calcium concentration, [Ca2+Ii, of 124 i 23 nh4 and a total cell calcium content, Ca,, of 12.8 t 6.8 nmoVmg cell protein. Proliferative zone chondrocytes have a [CaZ+Ii of 172 i 29 nM and a Ca, of 16.1 i 11.8 nmol/mg cell protein. Hypertrophic zone chondrocytes have a [Ca2+Ii of 273 i 49 nM and a Ca, of 35.8 * 16 nmol/rng protein. Chondrocytes isolated from the entire growth plate have a [Ca2+Ii of 132 2 29 nM and a Ca, of 13.2 * 2.5 nmol/mg cell protein. Chondrocytes isolated from noncalcifying hyaline cartilage have a [Ca2+Ii of 85 i 10 nM and a Ca, of 11.5 i 1.4 nmoVmg cell protein. There appears to be a correlation between intracellular calcium accumulation, an increase in the intracellular ionized calcium concentration, and the process of cartilage matrix mineralization.

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