Cytosolic gangliosides of rat brain: Their fractionation into protein-bound complexes of different ganglioside compositions

✍ Scribed by S. Sonnino; R. Ghidoni; A. Fiorilli; B. Venerando; Prof. G. Tettamanti

• Book ID: 102909471
• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 782 KB
• Volume: 12
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490120207

✦ Synopsis

The sialic acid moiety of rat brain cytosolic gangliosides was radiolabeled by intracranial injection of N-(3H)acetylmannosamine. Upon ammonium sulphate fractionation, Sepharose 6B gel filtration, and hydroxylapatite-cellulose chromatography, ganglioside-bound radioactivity of brain cytosolic extract followed the behavior of protein and not that of purified gangliosides. This indicates that cytosolic gangliosides occur as ganglioside-protein complexes.

By application of hydroxylapatite-cellulose column chromatography, fractions were obtained having different ganglioside composition. In particular, one fraction contained GMl, one GDla, and one GTlb with a ganglioside homogeneity better than 95% in each fraction. This indicates the occurrence in brain cytosol of a GM l-protein complex, a GDla-protein complex, and a GTlb-protein complex.