The chloroplast coupling factor (CF~) was dissociated into subunits by the freezing-thawing procedure in the presence of 0.5 M NaBr and the/3 subunit was purified by ion-exchange chromatography on a DEAE-cellulose column. The/3 subunit did not catalyze ATP hydrolysis either in the presence or in the
โฆ LIBER โฆ
Cytoplasmic inheritance of chloroplast coupling factor 1 subunits
โ Scribed by R. D. Durbin; T. F. Uchytil
- Book ID
- 104784019
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 203 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0006-2928
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โฆ Synopsis
An analysis of interspecific hybrids of Nicotiana spp. in which one of the parents was sensitive to tentoxin showed that this sensitivity was transmitted only through the female parent. Since tentoxin acts by selectively binding to the alpha,beta subunit complex of chloroplast coupling factor 1, the gene(s) specifying either one or both of these subunits is located in the cytoplasm.
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