Leydig cell number was evaluated quantitatively in testicular biopsies from post-pubertal cryptorchid patients and normal controls. For this quantitative evaluation we used the following method. This is based on the determination of the total number of Leydig cells, Leydig cell clusters and seminife
Cytoplasmic inclusion bodies in Leydig cells from the testes of postpubertal cryptorchid patients
β Scribed by Momokazu Gotoh; Koji Miyake; Hideo Mitsuya; Takeshi Hoshino; Kazuyori Yamada
- Book ID
- 114729997
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 608 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0105-6263
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A method is presented for the direct extraction of the recombinant protein Long-R 3 -IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells. Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extract
In previous parts of this study we developed procedures for the high-efficiency chemical extraction of soluble and insoluble protein from intact Escherichia coli cells. Although high yields were obtained, extraction of recombinant protein directly from cytoplasmic inclusion bodies led to low product