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Cytophotometric determination of DNA in cell nuclei

✍ Scribed by V. A. Benyush


Book ID
104947855
Publisher
Springer US
Year
1967
Tongue
English
Weight
508 KB
Volume
64
Category
Article
ISSN
0007-4888

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We describe a cytophotometric assay for unscheduled DNA synthesis (UDS) in asynchronously growing cells. Monolayer cultures of human HEp-2 and mouse MCa-11 cells were incubated with the carcinogen methylmethane sulfonate (MMS), as well as with hydroxyurea and (3H)thymidine. Slides were prepared, and