Cytomegalovirus proteins: II. Polypeptide composition of cytomegalovirus complement-fixing antigen
✍ Scribed by Milan Fiala; Douglas C. Heiner; John Murnane; Lucien B. Guze
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 395 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0146-6615
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Complement‐fixing (CF) antigen, prepared from cytomegalovirus (CMV)‐ infected cells and solubilized by urea and Tween‐20, was fractionated on agarose gel columns. Its molecular weight was 1.5−5 × 10^7^ daltons, and it contained 5 major peptides with molecular weights from 60,000–90,000 daltons. The peptide with a molecular weight of 70,000 daltons which was present in the solubilized CF antigen was absent in a soluble fraction from infected cells without CF activity and may be, therefore, important in this respect. The antigens present in the CF antigen did not elicit neutralizing antibody.