Cytolytic activity of human natural killer cell subpopulations isolated by four-color immunofluorescence flow cytometric cell sorting
✍ Scribed by Dr. Edward F. Srour; Tom Leemhuis; Laura Jenski; Robert Redmond; Jan Jansen
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 429 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
The natural killer activity and phenotypic properties of six different subpopulations of normal human peripheral blood lymphocytes obtained by fourcolor immunofluorescence cell sorting were examined. Phycoerythrin-conjugated CD16 and CD56 were used simultaneously to identify (CD16 + CD56 +) NK cells. The cells most effective in mediating NK cytolysis against K562 target cells were CD3-(16 + 56 +)57-8 + . Although most of the K562 killing was found in the CD3-(16+56+) groups of cells, a substantial degree of NK activity was detected in the CD3 + (16 + 56 + ) subpopulations of some individuals. The level of expression of CD57 and CDS was significantly higher on CD3 + (16 + 56 + ) than on CD3-(16+56+) cells.