Cytological analyses of a 14p+variant by means of N-banding and combinations of silver staining and chromosome bandings

✍ Scribed by Y. F. Lau; W. Wertelecki; R. A. Pfeiffer; F. E. Arrighi

• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 426 KB
• Volume: 46
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00278904

✦ Synopsis

An inherited human karyological variant (14p+) has been studied with a number of cytochemical techniques. The short arm of this variant chromosome 14 is nearly as long as the long arm, giving the chromosome a submetacentric to metacentric appearance. In conventionally Giemsa-stained preparations, maximum of three secondary constrictions can be observed the marker arm. The secondary constrictions are silver-positive in Ag-NOR preparations. However, the entire arm stains deeply in N-banded preparations. The 14p+ arm is also Q-negative, C-negative, G-negative, and R-positive with an almost homogeneous texture. The difference between N-banding and silver staining is interpreted as the result of gene activities of the ribosomal cistrons.