Cytokines in chronic inflammatory arthritis. III. Rheumatoid arthritis monocytes are not unusually sensitive to γ-interferon, but have defective γ-interferon–mediated HLA–DQ and HLA–DR induction
✍ Scribed by Ville Bergroth; Nathan J. Zvaifler; Gary S. Firestein
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 589 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
Macrophages present in the synovium and synovial fluid of patients with rheumatoid arthritis (RA) express large amounts of HLA-DR molecules on their surface, despite low levels of yinterferon (7-IFN) in the joint. To determine whether this apparent paradox is the result of increased sensitivity to y-IFN in RA, we compared concentrations of y I F N that induced HLA-DR and DQ on peripheral Mood monocytes of RA patients and normal donors, using fluorescenceactivated cell sorter analysis. Among normal donors, highly variable sensitivity to y-IFN was observed. Higher amounts of y-IFN were required to induce class 11 major histocompatibility complex molecules on RA monocytes versus normal monocytes. The maximum amount of HLA-DR that could be induced on RA and normal monocytes was similar; however, peak levels of HLA-DQ were significantly less in RA. Monocytes from patients with other forms of chronic inflammatory arthritis had intermediate HLA-DQ expression after 'y- IFN treatment. These data suggest that an increased sensitivity to y-IFN in RA does not account for the high level of HLA-DR expression in the joint. Also, a defect in HLA-DQ and HLA-DR induction by y I F N was observed.
Macrophages in the synovium and monocytes in the synovial fluid (SF) of patients with rheumatoid From the