Cytokine-induced expression of mRNAs for chemotactic factors in human synovial cells and fibroblasts

✍ Scribed by Pierre-André Bédard; Elaine E. Golds

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 932 KB
• Volume: 154
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041540227

✦ Synopsis

H3C 317 i P -A 5 ), and )omt Dr5eascs Ldboratory, Shrrners Hosprtal, MontrGal H3C; IA6 (f t C ), Quebec, Candda In response to interleukin 1 or tumor necrosis factor, human synovial cells and fibroblasts expressed several genes encoding known chemotactic factors or related proteins. Transcripts for interleukin 8 (IL-81, gro/MCSA, pAT 464, IP-I 0, PAT 744 and Monocyte Chcmotactic and Activating Factor (MCAF) accumulated rapidly in IL-1 and TNF-treated cells. The inhibition of protein synthesis led to the superinduction of IL-0 and gro/MGSA mRNAs in IL-I, but not in TNF-treated cells. Thus, IL-1 and TNF are likely to regulate the expression of these mRNAs by different mechanisms. Important cell-specific differences in mRNA accumulation characterized the expression of chemotactic factor genes. Moreover, only a subset of the same genes was activated in quiescent cells stimulated by serum. Therefore, genes encoding closely related proteins each had a distinct pattern of expression.

Continuous stimulation of fibroblasts and synovial cells with IL-1 resulted in high and prolonged expression of IL-8 and groiMGSA mRNAs. These results extend the list of chemotactic factor genes expressed by tnesenchymal cells in vitro and suggest a pivotal role for these cells in processes such as chronic inflammation. 0 I993 Wiley-Liss, Inc