Cytokine and adhesion molecule expression in SCID mice reconstituted with CD4+ T cells

✍ Scribed by Shigeyuki Kawachi; Zenichi Morise; Stephen R. Jennings; Elaine Conner; Adam Cockrell; F. Stephen Laroux; Robert P. Chervenak; Michael Wolcott; Henri Van Der Heyde; Laura Gray; Lan Feng; D. Neil Granger; Robert A. Specian; Dr. Matthew B. Grisham

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 1004 KB
• Volume: 6
• Category: Article
• ISSN: 1078-0998
• DOI: 10.1002/ibd.3780060304

✦ Synopsis

The objectives of this study were to quantify colonic cytokine and endothelial cell adhesion molecule (ECAM) expression in the colons of severe combined immunodeficient (SCID) mice reconstituted with different subsets of CD4+ T lymphocytes. We found that animals injected with CD45RBhigh but not CD45RB'"" T cells or phosphate-buffered saline (PBS) developed clinical evidence of colitis at 6-8 weeks following reconstitution, as assessed by loss of body weight, development of loose stools andor diarrhea, and histopathology. Concurrent with the onset of distal bowel inflammation was enhanced expression of a variety of Thl and macrophage-derived cytokines including interferon y, tumor necrosis factor-a, interleukin (1L)-1 p, IL-6, L-12, and IL-18 lymphotoxin-P. In addition, message levels and vascular surface expression of ICAM-I , VCAM-1, and MAdCAM-1 were all significantly enhanced in