Cytokeratin labeling of breast cancer cells extracted from paraffin-embedded tissue for bivariate flow cytometric analysis

✍ Scribed by Jeri K. Glogovac; Peggy L. Porter; Deborah E. Banker; Peter S. Rabinovitch

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 791 KB
• Volume: 24
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/(sici)1097-0320(19960701)24:3<260::aid-cyto9>3.0.co;2-l

✦ Synopsis

Flow cytometric analyses of DNA content and proliferative fractions have been found to be important prognostic indicators in a variety of human tumors. However, variability in reported results and interlaboratory differences in single-parameter DNA measurements have impeded the broader use of this methodology. Multiparameter DNA analysis, especially that which allows the ploidy and cell cycle

MATERIALS AND METHODS

Cell Preparation

Paraffin blocks were obtained from 178 patients with carcinoma of the breast. These specimens were submit-Address reprint requests to Peter s. Rabinovitch, Department of Pathology, Box 357470,