In vitro incorporation of tritium into n
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Françoise Doppler-Bernardi; Gary Felsenfeld
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Article
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1969
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Wiley (John Wiley & Sons)
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English
⚖ 540 KB
An exchange method is described for producing tritium-labeled native DNA in vitro with minimal physical damage to the DNA. Tritium-labeled calf thymus DNA prepared in this way has a specific activity of about 100 pCi/mmole of nucleotide (i.e., about 2 x 108 dpm/mmole). Sedimentation velocity a t neu