Cytogenetic analysis of 280 patients with multiple myeloma and related disorders: Primary breakpoints and clinical correlations
✍ Scribed by María J. Calasanz; Juan C. Cigudosa; María D. Odero; Carmen Ferreira; M. Teresa Ardanaz; Aurora Fraile; José L. Carrasco; Françesc Solé; Braulia Cuesta; Arturo Gullón
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 132 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Cytogenetic analysis of unstimulated short-term bone marrow cell cultures was performed on 280 patients with multiple myeloma and related disorders. In 65% of the cases, an additional short term B-cell stimulated culture was also examined. Chromosomally abnormal clones were found in 31% of the patients, 15% in Waldenstro ¨m macroglobulinemia, 25% in monoclonal gammopathies, 33% in multiple myeloma, and 50% in plasma cell leukemia. Three primary chromosomal breakpoints were recurrently involved: 14q32, 16q22, and 22q11. Structural rearrangements of chromosome 1 were the most frequent (26% of the abnormal cases), but always as a secondary change. Rearrangements of band 14q32 were found in 22% of the abnormal cases. Among the multiple myeloma patients who showed an abnormal karyotype, 33 (46%) were hyperdiploid, most frequently with 52-56 chromosomes, 29 patients (40%) were pseudodiploid, and the remaining 12 cases (14%) were hypodiploid. A highly significant relation was observed between the presence of an abnormal karyotype and the following clinical parameters: stage III (P 5 0.0001), bone marrow plasma cell infiltration greater than 30% (P 5 0.0001), presence of bone lesions (P 5 0.0009), and b2-microglobulin levels greater than 4 mg/L (P 5 0.0001).