Cytochrome P-450 expression and lipid peroxidation in gallbladder cancer

✍ Scribed by Manoj Pandey; Lal B. Sharma; Vijay K. Shukla

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 66 KB
• Volume: 82
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/jso.10206

✦ Synopsis

Abstract

Background and Objectives

Carcinoma of the gallbladder is the commonest neoplasm of the biliary tract; however, the etiology of this tumor remains unclear.

Methods

A case‐control study was carried out comprising 50 patients with gallbladder cancer and 50 patients with cholelithiasis. Cytochrome P‐450 estimation was carried out in the gallbladder tissue, and lipid peroxide product melonaldehyde (MDA) was measured in gallbladder bile. Statistical analysis was carried out by Student's t‐test, and one‐way analysis of variance (ANOVA). The Pearson correlation coefficient was calculated, and linear regression analysis was performed.

Results

The mean cytochrome P‐450 concentration was significantly higher in patients with carcinoma of the gallbladder than in controls (t = 3.9, P < 0.001; F = 15.2, P = 0.002). Similarly, the mean concentration of melonaldehyde was significantly higher among cancer patients than in patients with gallstones (t = 2.7, P < 0.003; F = 7.37, P = 0.0078). No significant correlation was observed between tissue MDA and cytochrome P‐450 levels (Pearson r = 0.1, P = 0.28).

Conclusions

The results of the present study show that the rise in concentration of MDA is independent of the rise in concentration of cytochrome P‐450; however, this is statistically insignificant. This could be due to biliary stasis leading to a rise in biliary concentration of melonaldeyde that is disproportionate to its production. J. Surg. Oncol. 2003;82:180–183. © 2003 Wiley‐Liss, Inc.