Cytochrome c dissolved in 1-allyl-3-methylimidazolium chloride type ionic liquid undergoes a quasi-reversible redox reaction up to 140°C
✍ Scribed by Kaori Tamura; Nobuhumi Nakamura; Hiroyuki Ohno
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 899 KB
- Volume
- 109
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Solubility of cytochrome c, a typical heme protein, in a various ionic liquids has been analyzed. The solubility has been discussed with polarity parameters of the ionic liquids. Both hydrogen bond basicity and dipolarity/polarizability of the ionic liquids were confirmed to be influential factors to control the solubilization of cytochrome c. Polar ionic liquids such as 1‐butyl‐3‐methylimidazolium chloride and 1‐allyl‐3‐methylimidazolium chloride solubilized cytochrome c at 80°C, and the dissolved cytochrome c was found to keep its redox activity in these ionic liquids. The redox response of the dissolved cytochrome c was detected in 1‐allyl‐3‐methylimidazolium chloride up to 140°C. Biotechnol. Bioeng. 2012; 109:729–735. © 2011 Wiley Periodicals, Inc.