Cytochrome c chimerae from natural and synthetic fragments: Significance of the biological properties

✍ Scribed by Carmichael J. A. Wallace; Giampietro Corradin; Fernando Marchiori; Gianfranco Borin

• Publisher: Wiley (John Wiley & Sons)
• Year: 1986
• Tongue: English
• Weight: 709 KB
• Volume: 25
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.360251107

✦ Synopsis

Analogs of natural proteins have application in basic research, in medicine, and increasingly, in industry. Of the many methods developed for the fabrication of modified proteins, semisynthesis has so far been the most productive [R. E. Offord (1983) Roc. Eur. Pep& Symp., 17 31-42]. The technique of fragment-condensation semisynthesis takes as its basic raw material the native protein; fragments derived from it are manipulated by sequential degradation and resynthesis before recombination. While gene manipuIation may overtake protein manipulation in general usefulness, certain types of analogs will remain accessible only by semisynthesis. Fragment condensation semisynthesis, as described above, has been used in preparing cytochrome c analogs [C. J. A. Wallace (1979) Proc. Am. Peptide Symp. 6, 609-6121. To overcome the difficulty of replacing amino acids far from convenient cleavage sites, a number of variants of the method have also been used. These include fragment-specific chemical modification [C.