Cytochemical study of Streptococcus agalactiae and macrophage interaction

✍ Scribed by Cristina Ferreira Teixeira; Neide Lemos Azevedo; Técia Maria Ulisses Carvalho; Jemima Fuentes; Prescilla Emy Nagao

• Book ID: 102333547
• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 262 KB
• Volume: 54
• Category: Article
• ISSN: 1059-910X
• DOI: 10.1002/jemt.1137

✦ Synopsis

Abstract

Light and electron microscopy were used to analyse the process of interaction of Streptococcus agalactiae (serotypes Ia, III, and V) with resident and activated mouse peritoneal macrophages. Transmission electron microscopy showed that adherence of the S. agalactiae serotype Ia, but not III and V serotypes, to the surface of activated macrophages triggers the respiratory oxidative burst as revealed by the presence of reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H]‐oxidase in the phagocytic vacuoles. Fusion of macrophage lysosomes with bacteria‐containing phagocytic vacuoles was observed in macrophages treated with Lucifer yellow as well as by localization of acid phosphatase for all serotypes. Microsc. Res. Tech. 54:254–259, 2001. © 2001 Wiley‐Liss, Inc.