Cytochemical Assay of Interferon Produced by Duck Hepatitis Virus
β Scribed by Sueltenfuss, E. A.; Pollard, M.
- Book ID
- 121169434
- Publisher
- American Association for the Advancement of Science
- Year
- 1963
- Tongue
- English
- Weight
- 495 KB
- Volume
- 139
- Category
- Article
- ISSN
- 0036-8075
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## Abstract Interferons have been evaluated extensively as candidate antiviral agents in hepadnaviral infection. We examined the effect of recombinant human interferonβΞ³ on duck hepatitis B virus replication in human hepatoma cells (Huh 7) transiently transfected with cloned duck hepatitis B virus
A simple method is described for the precise quantification of infectious duck hepatitis B virus (DHBV) in cell culture, using a radioimmunofocus assay (RIFA). Primary duck hepatocyte cell cultures were infected with serial dilutions of viral samples as for a plaque assay, but then maintained with l