Cytochalasin-E-Resistant Mutants ofCoprinus cinereus:Isolation and Genetic, Biochemical, and Cytological Analyses
β Scribed by Masashi Tsukamoto; Mari Yamamoto; Shigeru Tanabe; Takashi Kamada
- Book ID
- 115608846
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 837 KB
- Volume
- 20
- Category
- Article
- ISSN
- 1087-1845
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Mutants resistant to 200 micrograms/ml of the alkaloid lycorine (LYCR) in non-fermentable substrate were isolated after nitrosoguanidine mutagenesis. Tetrad analysis and growth of heterozygous (LYCR/lycS) diploids from two different mutants revealed that a single nuclear and dominant mutation is res
## Abstract A mutant (A204) of Chinese hamster ovary cells (CHOβK1), which is deficient in dihydroorotate (DHO) dehydrogenase (E.C. 1.3.3.1) activity, has been isolated by a replica plating procedure. The mutant does not show a requirement for exogenously added pyrimidines. Examination of intact ce