Cysteine 17 of recombinant human granulocyte-colony stimulating factor is partially solvent-exposed
β Scribed by Tsutomu Arakawa; Steven J. Prestrelski; Linda O. Narhi; Thomas C. Boone; William C. Kenney
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 610 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1573-4943
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Combinations of low dosages of purified recombinant human (rh) macrophagecolony stimulating factor (M-CSF; also termed CSF-1) and rh granulocyte-colony stimulating factor (G-CSF) were compared alone and in combination for their influence on the cycling rates and numbers of bone marrow and splenic gr
A new mutant protein of recombinant human granulocyte colony-stimulating factor (rhG-CSF) was produced for the studies on receptors for human G-CSF. The mutant protein [(Tyr1, Tyr3]rhG-CSF), the biological activity of which was almost equal to that of rhG-CSF, was prepared by the replacement of thre
To examine binding of recombinant human granulocyte-colony stimulating factor to myeloid cells, the factor was labeled with fluorescein isothiocyanate, and incubated with blood specimens, which were then analyzed by flow cytometry. Neutrophils demonstrated an increased fluorescence, while lymphocyte