CYR61 is a novel gene associated with temperature-dependent changes in fish metabolism as revealed by cDNA microarray analysis on a medaka Oryzias latipes cell line
✍ Scribed by Makoto Hirayama; Md. Nazmul Ahsan; Hiroshi Mitani; Shugo Watabe
- Book ID
- 102302882
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 448 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Abstract
A microarray comprising 3,514 cDNAs was constructed from a medaka EST library to elucidate the transcriptional responses associated with temperature shift from 25 to 15°C in a medaka cell line. Microarray analysis revealed that the mRNA levels of 313 clones were significantly different in at least one combination of different incubation periods up to 7 days at a given incubation temperature or between 25 and 15°C at a given incubation period (P < 0.05). These genes are known to be associated with various biological processes including morphogenesis, cell proliferation and response to stress. A number of genes encoding proteins which localize in extracellular areas were apparently up‐regulated at 15°C, whereas those localizing in intracellular areas were down‐regulated at this temperature. In addition, while a number of genes represented long‐term expression changes, only a few responded to short‐term inductions. A typical example was CYR61, a multifunctional matricellular signaling modulator, the mRNA levels of which increased after temperature shift from 25 to 15°C in 3 h, and then decreased rapidly to near the original level within 12 h. Another series of analyses by quantitative reverse transcription‐PCR revealed that the mRNA levels of CYR61 at 5°C were significantly higher even at 24 h after temperature shift compared to those of the cells successively maintained at 25°C. These analyses suggest that remodeling and reorganizing of extracellular structure of cells are important to offset the low temperature effect and CYR61 is considered to be a novel gene associated with temperature response in poikilotherms. J. Cell. Biochem. 104: 1297–1310, 2008. © 2008 Wiley‐Liss, Inc.