Cyclosporin A does not affect the in vitro induction of antigen-specific delayed-type hypersensitivity-mediating T cells

✍ Scribed by Babak Shidani; Iris Motta; Paolo Truffa-Bachi

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 411 KB
• Volume: 17
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830170222

✦ Synopsis

In vitro generation of T-delayed-type hypersensitivity effector cells in presence of cyclosporin A 291 Short paper Babak Shidani, Iris Motta and Paolo Truffa-Bachi Unit6 d'Immunophysiologie Cyclosporin A does not affect the in vitro induction of antigen-specific delayed-type hypersensitivitymediating T cells* MolCculaire, Institut Pasteur, Paris

The effect of cyclosporin A (CsA) on the in vitro induction of sheep red blood cell (SRBC)-specific T cells mediating the delayed-type hypersensitivity (DTH) reaction was studied. CsA (1 pglml) addition to the culture medium totally inhibits the in vitro anti-SRBC humoral response but does not interfere with the generation of anti-SRBC DTH-mediating T (TDTH) cells. The induction of TDW requires the presence of antigen and their expression is mediated selectively by the antigen used in the culture.

The TDTH cells generated are Lyt-1+,2-and their induction occurs with a similar efficiency in CsA-treated and untreated cultures: the frequency of the TD, cells increases by a factor of 10 to 20 during the 6-day incubation period. Lyt-2' suppressor cells are also generated in CsA-treated or untreated cultures. Our results suggest that a small fraction of T cells can be driven into the proliferative pathway by antigen even in presence of CsA.