-Cyclodextrin decreases cholera toxin binding to GM1-gangliosides
โ Scribed by Ermolinsky, B.; Peredelchuk, M.; Provenzano, D.
- Book ID
- 120256695
- Publisher
- Society for General Microbiology
- Year
- 2013
- Tongue
- English
- Weight
- 170 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0022-2615
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๐ SIMILAR VOLUMES
Ganglioside G M1 is the receptor for cholera toxin on cell surfaces, and the binding of cholera toxin to G M1 immobilized on microtitre plates has been reported previously by several authors as an assay for the toxin (G M1 -ELISA). This assay has been examined in detail. Results were independent of
Synthetic and natural amphiphiles, octyl glucoside, Nonidet P40, sodium dodecyl sulfate (SDS), gangliosides GM1 and GD1a, interact with cholera toxin (CLT) and with its active region (promoter A). The formation of CLT-amphiphile complex leads to inhibition of ADP-ribosyltransferase activity, a chara