Cyclodextrin-catalyzed oxidation of glutathione in solution and in an ion trap

✍ Scribed by Weixing Sun; Junqiu Liu; Mei Cui; Fengrui Song; Shuying Liu

• Book ID: 101238021
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 70 KB
• Volume: 13
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19990530)13:10<950::aid-rcm592>3.0.co;2-5

✦ Synopsis

Incubated solutions containing glutathione (GSH) and aor b-cyclodextrins (CDs) were analyzed using electrospray mass spectrometry and tandem mass spectrometry. The results suggest that both CDs can catalyze oxidation of GSH to the oxidized glutathione (GSSG). The collision-induced dissociation (CID) of the 1:1 and 1:2 (CD/GSH) and 1:1 (CD/GSSG) complexes reveals the strong interactions of the CDs with the peptides tested. The 1:2 (CD/GSH) complex is considered to be the oxidation reaction intermediate, which indicates that the three-dimensional structure of the complexed two GSHs in CD complexes is different from that of the proton-bound GSH dimer. The oxidation product, GSSG, is also observed in the CID spectrum of the singly charged 1:1 (CD/GSH) complex, suggesting that a complex ion-complex ion reaction occurs by forming a doubly charged complex dimer, as a result of the ability of ion trap to accumulate and activate ions. The observations indicate that ion trap mass spectrometry can be used to explore cyclodextrincatalyzed reactions and to carry out complex gaseous chemistry research.